Quantifying Siglec-sialylated ligand interactions: a versatile 19F-T2 CPMG filtered competitive NMR displacement assay

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dc.contributor.affiliation000000041762408X
dc.contributor.affiliationChemical Glycobiology Lab, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia, Derio, 48160, Spain; Glycotechnology Laboratory, CIC biomaGUNE, Paseo Miramon 194, San Sebastian, 20014, Spain; Departments of Molecular Medicine and Immunology & Microbiology, The Scripps Research Institute, 10550 North Torrey Pines Road, San Diego, 92037, CA, United States; Department of Organic Chemistry, Universidad Autónoma de Madrid, C/Francisco Tomás y Valiente 7, Madrid, 28049, Spain; Institute for Advanced Research in Chemical Sciences (IAdChem), Universidad Autónoma de Madrid, C/Francisco Tomás y Valiente 7, Madrid, 28049, Spain; Instituto Madrileño de Estudios Avanzados (IMDEA)-Nanociencia, C/Faraday 9, Madrid, 28049, Spain; CIBER-BBN, Paseo Miramon 194, San Sebastian, 20014, Spain; Ikerbasque, Basque Foundation for Science, Bilbao, Spain; Department of Organic & Inorganic Chemistry, Faculty of Science and Technology, University of the Basque Country, EHU-UPV, Bizkaia, Leioa, 48940, Spain; Centro de Investigacion Biomedica en Red de Enfermedades Respiratorias, Madrid, 28029, Spainen
dc.contributor.authorAtxabal U.
dc.contributor.authorFernández A.
dc.contributor.authorMoure M.J.
dc.contributor.authorSobczak K.
dc.contributor.authorNycholat C.
dc.contributor.authorAlmeida-Marrero V.
dc.contributor.authorOyenarte I.
dc.contributor.authorPaulson J.C.
dc.contributor.authorEscosura A.D.L.
dc.contributor.authorTorres, Tomás
dc.contributor.authorReichardt N.C.
dc.contributor.authorJiménez-Barbero J.
dc.contributor.authorEreño-Orbea J.
dc.date.accessioned2024-09-05T10:43:19Z
dc.date.available2024-09-05T10:43:19Z
dc.date.issued2024
dc.format.mimetypeapplication/pdf
dc.identifier.doi10.1039/d4sc01723d
dc.identifier.urihttps://hdl.handle.net/20.500.12614/3741
dc.journal.titleChemical Science
dc.language.isoen
dc.relation.projectIDTEMP"This work was supported by the European Research Council (788143-RECGLYCANMR to J. J.-B) and the Marie-Sk\u0142odowska-Curie actions (ITN Glytunes grant agreement no. 956758 to J. E.-O"," and ITN BactiVax under grant agreement no. 860325 to U. A.). The NMR spectrometers are part of the National NMR Network (PTNMR) and are partially supported by Infrastructure Project No. 22161 (co-financed by FEDER through COMPETE 2020, POCI and PORL and FCT through PIDDAC). JJB acknowledge to the European commission for the COST Action 18132 \u2013 GLYCONANOPROBES and the GLYCOTWINNING project and CIBERES, an initiative of Instituto de Salud Carlos III (ISCIII, Madrid, Spain). We thank Agencia Estatal de Investigaci\u00F3n of Spain for grants PID2019-107770RA-I00 (J. E.-O.), PID2020-119306GB-100 (A. d. l. E.), and the Severo Ochoa Center of Excellence Accreditation CEX2021-001136-S, all funded by MCIN/AEI/10.13039/501100011033. T. T. acknowledges financial support from the Spanish MCIN/AEI/10.13039/501100011033 (PID2020-116490GB-I00), the Comunidad de Madrid and the Spanish State through the Recovery, Transformation and Resilience Plan [\u201CMateriales Disruptivos Bidimensionales (2D)\u201D (MAD2D-CM) (UAM1)-MRR Materiales Avanzados], and the European Union through the Next Generation EU funds. IMDEA Nanociencia acknowledges support from the \u201CSevero Ochoa\u201D Programme for Centres of Excellence in R&D (MINECO, Grant SEV2016-0686)."
dc.rightsAtribucion-NoComercial-SinDerivadas 3.0 España*
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.titleQuantifying Siglec-sialylated ligand interactions: a versatile 19F-T2 CPMG filtered competitive NMR displacement assay
dc.typeresearch article
dc.type.hasVersionVoR

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