------------------- GENERAL INFORMATION ------------------- Related publication (journal paper, book, etc.): Alberto Martin-Asensio, Irene Pardo, Lucía Pardinas, Edgar Eduardo Villalobos-Portillo, Demian Pardo, Gorka Salas, Milagros Castellanos, Álvaro Somoza, Jaime J. Hernández, Isabel Rodríguez. Real-time Tracking of Drug Nanocarrier Diffusion in Biological Media within Microfluidic Devices Using Synchrotron X-Ray Fluorescence Imaging. Date(s) of data collection: 2024 Geographic location of data collection: IMDEA Nanociencia, Madrid; ALBA Synchrotron, Barcelona General description: Data reported in the article 'Real-time Tracking of Drug Nanocarrier Diffusion in Biological Media within Microfluidic Devices Using Synchrotron X-Ray Fluorescence Imaging' -------------------------- SHARING/ACCESS INFORMATION -------------------------- Licenses/restrictions placed on the data, or limitations of reuse: N.A. Links/relationships to previous or related data sets: N.A. -------------------- DATA & FILE OVERVIEW -------------------- File list: Figure 1 3D CAD Chip Design STL, Figure 1 3D CAD Chip Design F3D, Fluorescence Data, Map_Data_Au, Map_Data_Fe, Au@Fe3O4_PEG, AuNPs_13nm_PEG3K, AuNPs_13nm_Pept, AuNPs_13nm_PolyT22 and AuNPs_22nm_PolyT22 Maps Data, Mean_diffusion_values, XRF Maps analysis. ***Relationship between files: The file XRF Maps análisis.py contains the code for the data analysis, as described in the article’s main text. This code analyzes the raw data as obtained directly from the ALBA synchrotron facility. The raw data used to obtain the results shown in the article is provided in this repository and stored in the Map Data folder. Inside this folder, the data obtained for each nanoparticle is stored in a specific with the sample name as follows: - Map Data : o AuNPs_22nm_PolyT22  AuNPs_22nm_PolyT22_PBS_1_0.dat  AuNPs_22nm_PolyT22_PBS_2_0.dat  AuNPs_22nm_PolyT22_PBS_3_0.dat  AuNPs_22nm_PolyT22_PBS_4_0.dat  AuNPs_22nm_PolyT22_PBS_5_0.dat  AuNPs_22nm_PolyT22_PBS_6_0.dat  AuNPs_22nm_PolyT22_PBS_7_0.dat - AuNPs_13nm_PolyT22  AuNPs_13m_PolyT22_PBS_54nM_1_0.dat  AuNPs_13m_PolyT22_PBS_54nM_2_0.dat  AuNPs_13m_PolyT22_PBS_54nM_3_0.dat  AuNPs_13m_PolyT22_PBS_54nM_4_0.dat  AuNPs_13m_PolyT22_PBS_54nM_5_0.dat  AuNPs_13m_PolyT22_PBS_54nM_6_0.dat  AuNPs_13m_PolyT22_PBS_54nM_7_0.dat - AuNPs_13nm_Pept  AuNPs_13m_Pepido_PBS_1_0.dat  AuNPs_13m_Pepido_PBS_2_0.dat  AuNPs_13m_Pepido_PBS_3_0.dat  AuNPs_13m_Pepido_PBS_4_0.dat  AuNPs_13m_Pepido_PBS_5_0.dat  AuNPs_13m_Pepido_PBS_6_0.dat  AuNPs_13m_Pepido_PBS_7_0.dat - AuNPs_13nm_PEG3K  AuNPs_13m_PEG3K_PBS_1_0.dat  AuNPs_13m_PEG3K_PBS_2_0.dat  AuNPs_13m_PEG3K_PBS_3_0.dat  AuNPs_13m_PEG3K_PBS_4_0.dat  AuNPs_13m_PEG3K_PBS_5_0.dat  AuNPs_13m_PEG3K_PBS_6_0.dat  AuNPs_13m_PEG3K_PBS_7_0.dat - Au@Fe3O4_PEG  LP10_PEGCOOH_PBS_1_0.dat  LP10_PEGCOOH_PBS_2_0.dat  LP10_PEGCOOH_PBS_3_0.dat  LP10_PEGCOOH_PBS_4_0.dat  LP10_PEGCOOH_PBS_5_0.dat  LP10_PEGCOOH_PBS_6_0.dat  LP10_PEGCOOH_PBS_7_0.dat Files are index 1 to 7 representing the acquisition order, with a time interval of 20 mins. As such, index 1 corresponds to t = 0 min, index 2 corresponds to t = 20 min, index 3 corresponds to t = 40 min and so on. The folder Figure 1 contains the data shown in the article’s Figure 1. It contains the files with the chip’s 3D design data in two separate files: - Figure 1 3D CAD Chip Design F3D.f3d: Autodesk Fusion 360’s native format, to open the 3D design with said program. - Figure 1 3D CAD Chip Design STL.stl: STL file with the design, used to print the device and that can be opened and visualized with a generic 3D visualization program. A free option is Blender 3D, using the Import-Export: STL format built-in add-on. It also contains the fluorescence maps shown in Figure 1: - Fluorescence Data.dat: Raw data obtained from the synchrotron. - Map_Data_Au.txt/Map_Data_Fe.txt: txt file containing the fluorescence image data as extracted by the python code. For easy visualization, these images can be opened with Fiji/ImageJ by selecting File -> Import -> Text image The file Mean_diffusion_values.txt contains the mean and standard deviation data shown in Figures 2-4. ***End 'Relationship between files' Formats: .stl, .f3d, .dat, .txt, .py Version: N.A. -------------------------- METHODOLOGICAL INFORMATION -------------------------- Description of methods used for *collection/generation* of data: X-ray Fluorescence Microscopy Methods for *processing* the data: Python 3.11 custom written software Software- or Instrument-specific information needed to *access/open/interpret* the data, including software and hardware version numbers: Autodesk Fusion 360 (v.2703.1.20), any software capable of opening stl files (i.e. Blender), Windows Notepad, Spyder IDE (v. 5.4.3) or any Python interpreter. Standards and calibration information: N.A. Environmental/experimental conditions: Experimental Hutch set-up (described in manuscript). Data taken at room temperature Describe any quality-assurance procedures performed on the data: N.A. -------------------------- DATA-SPECIFIC INFORMATION -------------------------- Variable list, defining any abbreviations, units of measure, codes or symbols used: D: Diffusion Coefficient (um^2/s)